Mot1 removal partially restores wild-type transcription in Med16-AID cells.

(A) Western blots showing the kinetics of Mot1-AID and Med16-AID upon 3-IAA treatment of double-degron cells. (B) Boxplots of log2 fold changes in nsRNA levels of transcripts produced from Med16-down and Med16-up genes for the Med16-AID and Mot1/Med16-AID 3-IAA versus DMSO comparisons. Statistical differences between groups were assessed by pairwise Wilcoxon rank-sum test with Holm correction for multiple testing. (C) Average plots of TBP ChEC-seq signal (≤80 bp fragments) from Med16-AID cells treated with 3-IAA or DMSO and free MNase from WT cells (MNase) in a 2 kb window centered around the TSSs of Med16-down and Med16-up genes.