Additional file 2: of Prenatal influenza vaccination rescues impairments of social behavior and lamination in a mouse model of autism

Figure S1. Effects of maternal VAC and MIA on cell apoptosis and proliferation in the developing neocortex at E18.5. (A) Low-magnification image of CC3+ cells (red). (B-E) High-magnification images of CC3+ cells (red). All nuclei are labeled with Hoechst (blue). Scale bar, 50 μm. (F) Low-magnification image of PCNA+ cells (red) showed that a majority of PCNA+ cells were located in the VZ/SVZ. (G) A representative blot of CC3 and PCNA is shown. Equal loading of proteins is illustrated by the β-actin bands. (H) The group densitometry analysis of CC3 protein. There was a main effect of MIA (F1,12 = 26.065, p < 0.001) and post hoc test showed increases in MIA (p = 0.003) and VAC+LPS (p = 0.004) mice compared with controls. (I) The group densitometry analysis of PCNA. There was a main effect of MIA (F1,12 = 14.42, p = 0.003) and post hoc test showed increases in the MIA group (p = 0.003), but VAC pretreatment had a trend toward preventing this effect (p = 0.288). n = 4 mice/group; **p < 0.01 (two-way ANOVA and Bonferroni post hoc test). The results are all shown as the mean + s.e.m. Scale bar, 50 μm in A-E, 500 μm in F. (TIF 2162 kb