Additional file 2: of Combination of alpha-synuclein immunotherapy with anti-inflammatory treatment in a transgenic mouse model of multiple system atrophy

Morphological analysis of striatal microglia by Iba1 immunostaining. (A) Representative high-magnification images of Iba1 immunostaining in the striatum of non-tg and MBP-α-syn tg mice treated with LV-control or LV-CD5-D5-ApoB, and vehicle or lenalidomide. Inverted images were used for quantification purposes. (B) Quantification of microglial ramification as measured by the number of branches per cell. (C) Microglial soma size as measured by the maximum soma diameter. A minimum of 20 cells were quantified per animal. Results are presented as average ± SEM. * p < 0.05, and *** p < 0.001 when comparing vehicle/LV-control-treated non-tg mice to vehicle/LV-control-treated tg mice. ## p < 0.01 and ### p < 0.001 when comparing vehicle/LV-control-treated tg mice to single or multi-treated tg mice. Scale bar = 20 μm. (TIF 1825 kb