Additional file 3: Figure S2. of α-Synuclein vaccination modulates regulatory T cell activation and microglia in the absence of brain pathology

MHC II, CD4, and mouse IgG immunohistochemistry. A, B Representative photomicrographs of SN brain sections stained for MHC II. C–E Representative photomicrographs of brain sections stained for CD4. No CD4 staining corresponding to T cells was observed throughout the brain in any of the groups, however sporadic CD4+ microglia was found in the adjuvant (A) and α-syn low dose (B) groups. Additionally, sporadic CD4+ immunostaining associated to blood vessels was found in the α-syn high dose group (C). F&G Representative pictographs of brain sections stained for mouse IgG. Only in the α-syn high-dose group’s hippocampus was sporadic mouse IgG staining observed (F); however, blood vessels in all groups stained positive for IgG (G). A, C, D & F: ×20 magnification, scale bar in E, 10 μm. B, E, G: ×10 magnification, scale bar in D, 100 μm. (PDF 2.38 mb