Supplemental Figure 4

Supplemental Figure 4. NUPR1 is present in a majority of somatolactotropes. A representative immunofluorescence microscopy image of WT female mouse pituitary cells that were disaggregated and immuno-stained for NUPR1, GH, and PRL. Multiple somatotropes (white arrows) can be seen which stain strongly for NUPR1, along with a single somatolactotrope (GH+/PRL+) (red arrow) that also is positive for NUPR1. Two GH-/PRL- cells are also visible which stain much more weakly for NUPR1. In this experiment, a total of 50 somatolactotropes were identified (out of approximately 500 cells imaged) and assayed for NUPR1 expression. Of these 50 somatolactotropes, 27 (54%) were positive for NUPR1.