Supplemental Figure 2

Supplemental Figure 2. Immunofluorescence microscopy of GH-eGFP pituitary cells demonstrating that GFP is selectively expressed in somatotropes. Pituitaries from GH-eGFP mice were disaggregated and co-stained for DAPI, GFP, GH, and PR,L, as indicated in each frame. The red arrows indicate three GFP+ cells which are also GH+. The white arrow indicates a PRL+ cell which is negative for GFP and GH. The Merge frame combines DAPI along with GFP (green), GH (red), and PRL (white). A total of 200 somatotropes and 200 lactotropes (identified by the expression of GH or PRL, respectively) were assayed for GFP expression. Out of 200 somatotropes, 184 (92%) were GFP positive. Out of 200 lactotropes, 2 (1%) were GFP positive. These data demonstrate robust somatotrope specificity for the GH-eGFP line. Bar, 5 um.