Supplemental Figure 4

Supplemental Figure 4. NUPR1 is present in a majority of somatolactotropes. A representative immunofluorescence microscopy image of WT female mouse pituitary cells that were disaggregated and stained for NUPR1, GH, and PRL. Here, multiple somatotropes (white arrows) can be seen which stain strongly for NUPR1, along with a single somatolactotrope (GH+/PRL+) (red arrow) that also is positive for NUPR1. Two GH-/PRL- cells are also visible which stain much more weakly for NUPR1. In this experiment, a total of 50 somatolactotropes were counted and assayed for NUPR1 expression. Of the 50 somatolactotropes, 27 (54%) were positive for NUPR1.