Correlation between BCAT1 gene expression and methylation.

3a)– 3c) show the significant inverse correlation between gene expression for one probe on BCAT1 (226517_at) and methylation at three separate CpG sites on BCAT1 (cg09800500, cg16490209 and cg07479001). Negative correlation is highlighted by the linear fit (dashed line) with corresponding correlation coefficients (r values) and statistical significance. 3d) Correlation between gene expression at the previously mentioned BCAT1 probe (226517_at) and DNA methylation improves when the three signficant CpG sites are combined (summed) for analysis. 3e) Two gene expression probes on BCAT1 (226517_at and 225285_at) are well correlated with one another. For all figures, normalized gene expression and methylation M values are shown. For 3a)- 3c), each point represents a paired gene expression and methylation sample. M values are inversely correlated with gene expression values as expected.