W for wild type lipase; M1 for mut1 and M2 for mut 2 lipases. The mid-point of fluorescence transition or where 50% relative fluorescence is achieved is the T_m of the enzymes. The calculated T_m for wild type, mut 1 and mut 2 were: T_m1: 59oC, T_m2: 63oC, T_m3: 66oC.</p
International audienceLip2 lipase from Yarrowia lipolytica is a very promising lipase with many pote...
The substitutions of the amino acid at the predetermined critical point at the C-terminal of L2 lipa...
<p>The temperature dependence of excess molar heat capacity of the wild-type <i>Mt</i>-NDPK (in red)...
Error bars are the standard deviations of experiments done independently and in triplicates.</p
<p>Stability of recombinant WT and Δ2 to Δ10 mutant <i>α</i>Gal at 30°C (a), 40°C (b), and 50°C (c) ...
In order to understand the molecular basis of cold adaptation, we have used directed evolution to tr...
<p>Residual lipase activity after thermal incubation for 30 minutes at concentrations of (A) 1 µM an...
<p>SAs were calculated from the velocity slope obtained for 10 minutes using 10 µg of enzymes. Resul...
<p>The optimal temperatures (a), enzyme inactivation curves at 60°C (b) and kinetic stability curves...
Engineering proteins to enhance thermal stability is a widely utilized approach for creating industr...
<p>The fluorescence intensity of the bound FMN is used to monitor the protein denaturation. As can b...
Lipases specifically thermostable lipases which are origin from microbial are desirable commercially...
AbstractDirected evolution by error-prone PCR was applied to stabilize the cold-active lipase from P...
Graph showing difference in RMSF between mut 1, mut 2 and wild type lipase at A 320K, B 330K, C 350K...
<p>(A) Activity of purified FPS1S (open symbols) and FPS2 (closed symbols) was measured after incuba...
International audienceLip2 lipase from Yarrowia lipolytica is a very promising lipase with many pote...
The substitutions of the amino acid at the predetermined critical point at the C-terminal of L2 lipa...
<p>The temperature dependence of excess molar heat capacity of the wild-type <i>Mt</i>-NDPK (in red)...
Error bars are the standard deviations of experiments done independently and in triplicates.</p
<p>Stability of recombinant WT and Δ2 to Δ10 mutant <i>α</i>Gal at 30°C (a), 40°C (b), and 50°C (c) ...
In order to understand the molecular basis of cold adaptation, we have used directed evolution to tr...
<p>Residual lipase activity after thermal incubation for 30 minutes at concentrations of (A) 1 µM an...
<p>SAs were calculated from the velocity slope obtained for 10 minutes using 10 µg of enzymes. Resul...
<p>The optimal temperatures (a), enzyme inactivation curves at 60°C (b) and kinetic stability curves...
Engineering proteins to enhance thermal stability is a widely utilized approach for creating industr...
<p>The fluorescence intensity of the bound FMN is used to monitor the protein denaturation. As can b...
Lipases specifically thermostable lipases which are origin from microbial are desirable commercially...
AbstractDirected evolution by error-prone PCR was applied to stabilize the cold-active lipase from P...
Graph showing difference in RMSF between mut 1, mut 2 and wild type lipase at A 320K, B 330K, C 350K...
<p>(A) Activity of purified FPS1S (open symbols) and FPS2 (closed symbols) was measured after incuba...
International audienceLip2 lipase from Yarrowia lipolytica is a very promising lipase with many pote...
The substitutions of the amino acid at the predetermined critical point at the C-terminal of L2 lipa...
<p>The temperature dependence of excess molar heat capacity of the wild-type <i>Mt</i>-NDPK (in red)...
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