GFP::BRC-1 and BRD-1::GFP are expressed in the germ line and respond to stalled/collapsed replication forks.

A) GFP fluorescence of whole worms expressing GFP::BRC-1, BRD-1::GFP, or no GFP (WT). Dashed line denotes germ line with arrows to indicate GFP fluorescence; blue arrows denote GFP signal in embryos; gut granules auto-fluoresce. Scale bar = 100μm. B) Schematic of the spatiotemporal organization of the hermaphrodite germ line with meiotic stages indicated. C) Proliferating germ cells (premeiotic) expressing GFP::BRC-1 or BRD-1::GFP (green), stained with antibodies against RAD-51 (red), and counterstained with DAPI (blue) in the absence (-HU) and presence of 5mM hydroxyurea (+HU). Blue arrows denote co-localization between GFP and RAD-51. Images are projections through half of the gonad. Scale bar = 5μm. D) Pearson’s Correlation Coefficient (PCC) measurements between RAD-51 and GFP::BRC-1 or BRD-1::GFP in the absence and presence of HU. 95% Confidence Intervals are shown. *** p< 0.0001, Mann-Whitney test; n = 10 nuclei.