Analysis of DAF-28::GFP and ss::GFP secretion.

<p>A) Quantification of DAF-28::GFP secretion defects in control and experimental RNAi worms. Error bars are +/− SE for three individual experiments. B) Paired DIC (top) and fluorescence (bottom) images of <i>daf-28::gfp</i> expressing worms exposed to control RNAi (top) or <i>ykt-6(RNAi)</i> (bottom). Arrows indicate coelomocytes. No DAF-28::GFP accumulation is seen in the coelomocytes of the <i>ykt-6(RNAi)</i> animal shown here. C) Paired DIC (left) and fluorescence (right) images of two <i>ykt-6</i>(RNAi) worms that did not secrete <i>daf-28::gfp</i>. DAF-28::GFP protein now accumulates around the gonad. n = intestinal nucleus. The anterior/posterior axis is indicated. D) Overlay of DIC and fluorescence images of worms with control RNAi and RNAi against indicated genes to test for the secretion and uptake of ss::GFP. In all cases GFP accumulates only in the coelomocytes (green cells).