BRC-1 promotes the stability of RAD-51 filaments when crossover formation is impaired.

A) Schematic of potential outcomes of IR-induced RAD-51 (red) over time in brc-1(tm1145) brd-1(dw1); spo-11; syp-2(RNAi) worms: a defect in RAD-51 loading would result in loss of the “dark zone” (circle) by 12 hrs (left), while a defect in RAD-51 stabilization would manifest in the maintenance of the dark zone over 12 hrs (right). B) Projections of whole germ lines at indicated times after IR treatment in brc-1(tm1145) brd-1(dw1); spo-11; syp-2(RNAi) (left) and spo-11; syp-2(RNAi) (right). DAPI (blue), RAD-51 (red). A minimum of 3 germ lines were imaged for each condition. Scale bar = 30 μm.