BRC-1 and BRD-1 are inter-dependent for localization.

Qianyan Li (5920223)
Takamune T. Saito (143217)
Marina Martinez-Garcia (824306)
Alison J. Deshong (555351)
Saravanapriah Nadarajan (141726)
Katherine S. Lawrence (322618)
Paula M. Checchi (207456)
Monica P. Colaiacovo (5920226)
JoAnne Engebrecht (207461)

Open link

Publication date

November 2018

DOI

10.1371/journal.pgen.1007701.g003

Publisher

Public Library of Science (PLoS)

Abstract

A) Co-localization between BRD-1::GFP (green) and TagRFP-T::BRC-1 (red) at late pachytene in live worms. Scale bar = 10 μm. B) Stills of germline nuclei from live worms expressing GFP::BRC-1 and mCherry::Histone H2B (mCherry::his-58; gfp::brc-1); merge and GFP channel; top two panels, respectively. GFP::BRC-1 expression in brd-1(ok1623) mutant at indicated meiotic stages. Bottom two panels show BRD-1::GFP localization in wild type and the brc-1(xoe4) mutant. Images are projections through half of the gonad. TZ = transition zone; EP = early pachytene; MP = mid pachytene; LP = late pachytene; DP = diplotene; DK = diakinesis. Scale bar = 5 μm. C) Immunoblot of whole worm extracts from indicated worms probed with anti-GFP and α-tubulin antibodi...