BCL-7 is involved in the asymmetric localization of Wnt components in <i>Caenorhabditis elegans</i>.

<p><b>A–D</b>: Nomarski (<b>A, C</b>) and GFP (<b>B, D</b>) images of wild type (<b>A, B</b>) and <i>tm5268</i> (<b>C, D</b>) L2-stage hermaphrodites carrying a <i>wrm-1::gfp</i> reporter (<i>osIs5</i>) at pre-division states. Seam cells are traced with dotted ovals (<b>A, C</b>). <b>E–H</b>: Nomarski (<b>E, G</b>) and GFP (<b>F, H</b>) images showing the localization of WRM-1::GFP in two daughter cells of wild-type or <i>tm5268</i> L2 hermaphrodites at post-divisions. The pairs of daughter cells are outlined with dotted ovals. <b>I</b>: Frequency of the localization patterns of WRM-1::GFP. The equal and unequal signs indicate the relative intensities of nuclear WRM-1::GFP between two daughter cells. <b>J–M</b>: Nomarski (<b>J, L</b>) and GFP (<b>K, M</b>) images showing the localization of GFP::POP-1 (<i>qIs74</i>) in two daughter cells of wild-type (<b>J, K</b>) or <i>tm5268</i> (<b>L, M</b>) L1 hermaphrodites in post-division states. The pairs of daughter cells are outlined with dotted ovals. Asterisks indicate nuclei with stronger expression of GFP. <b>N</b>: The frequency of the localization patterns of GFP::POP-1. The equal and unequal signs indicate the relative intensities of nuclear GFP::POP-1 between two daughter cells. <b>O–R</b>: Nomarski (<b>O, Q</b>) and GFP (<b>P, R</b>) images showing the localization of GFP::POP-1 (<i>qIs74</i>) in SGPs of wild-type (<b>O, P</b>) or <i>tm5268</i> (<b>Q, R</b>) early L1 hermaphrodites. SGPs are outlined with dotted ovals. <b>S</b>: The frequency of the localization patterns of GFP::POP-1. The equal and unequal signs indicate the relative intensities of nuclear GFP::POP-1 between two daughter cells. Anterior is oriented toward the left, and ventral is oriented toward the bottom. <b>T</b>: Percentages of worms with the aberrant POP-1 asymmetry in both Z1 and Z4 cells, either Z1 or Z4 cells, and no cells in one worm between wild type and <i>bcl-7</i> mutant worms. Numbers of examined samples were more than 30 for all analyses. Scale bar = 25 µm.