Activity of GLO-1(D132A) in gut granule protein localization.

(A-D) 1.5-fold stage embryos expressing GFP::GLO-1(D132A) were stained with antibodies to PGP-2 and LMP-1. Embryos were imaged with confocal microscopy, single optical sections are shown, and white arrows in the insets label organelles containing the gut granule protein PGP-2. Black arrowheads flank the intestine. (E-F) SQUASSH software was used to calculate Csize(PGP-2LMP-1 or GFP::GLO-1/PGP-2), which represents the proportion of total PGP-2 area in each embryo that also contained LMP-1 (E) or GLO-1::GFP (F) signals. Each data point represents the Csize of an individual embryo and at least 5 embryos of each genotype were scored. The mean level of colocalization per embryo is plotted, bars represent the 95% confidence intervals, and** indicates p≤0.005, * indicates p≤0.05, and ns indicates p>0.05, by one way ANOVA followed by a Tukey-Kramer test.