GLO-1 localization is disrupted in <i>ccz-1(-)</i> and <i>glo-3(-)</i> mutants.

(A-H) GLO-1::GFP in living 1.5-fold stage embryos was imaged by wide field microscopy. Embryos were identically imaged and line scan fluorescence intensity centered on 5 puncta (examples are marked with white arrows) from each of 5 different embryos (scans from a single embryo are the same color) was measured. The intestine is flanked by black arrowheads. (I) The background was subtracted from the maximum intensity of each profile in A-H and the mean difference is plotted. Bars are the 95% confidence intervals and ** indicates p≤0.001 by one way ANOVA comparing the mutants to wild type. (J-K) Bean stage embryos expressing CDF-2::GFP were stained with anti-GLO-1 antibodies and imaged with confocal microscopy. (J) CDF-2::GFP marked organelles in wild type contained GLO-1 (white arrows in insets). (K) GLO-1 was lacking from these organelles in glo-3(kx38) mutants (white arrowheads in insets). (L) SQUASSH software was used to calculate the area of CDF-2::GFP organelles that contained GLO-1. The mean is plotted and bars are the 95% confidence intervals and ** indicates p≤0.001 by one way ANOVA comparing the glo-3(-) mutant to wild type.