The expression and localization of GLO-2.

<p>(A–D) <i>glo-2::gfp</i> was expressed under the control of the operon internal promoter immediately upstream of <i>glo-2</i>. (A–B) GLO-2::GFP expression was first detected in intestinal precursors (marked with asterisks) at the E⁴ stage of embryogenesis. (C–D) At the 1.5-fold stage, GLO-2::GFP was expressed in intestinal cells (between black arrowheads) and was localized to the cytoplasm. (E–F) Wild-type and <i>glo-2(zu455)</i> pretzel-stage embryos stained with anti-GLO-2 antibodies. <i>glo-2(zu455)</i> embryos lacked staining of hypodermal organelles by GLO-2 antibodies (white arrows) however they retained staining of P-granules (black arrows). GLO-2 antibodies did not stain prominent organelles within intestinal cells (between black arrowheads). Single focal planes are shown in A–D and maximum intensity projections of 1.25 micron Z-stacks are shown in E–F.