EFF-1(S632/634/654A)::GFP localization patterns are similar to wild-type EFF-1::GFP <i>in vivo</i>.

<p>Time-lapse images of EFF-1(S632/634/654A)::GFP show spatiotemporal localization to fusion competent ventral (A) cell borders (arrow) in the absence of phosphorylatable residues within putative 14-3-3 binding sites compared to wild-type EFF-1::GFP (B). One-micron-spaced image stacks were captured every 2.5 minutes using widefield (A) or confocal (B) microscopy, and maximum intensity Z-projections of the ventral surface were rendered. In 100% of the mutant embryos (n = 3), the same pattern of junctional localization is seen as for wild-type EFF-1::GFP (B) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146874#pone.0146874.ref038" target="_blank">38</a>]. Anterior is up and posterior is down. Times shown are in minutes.