Generation of GLO-1-positive lysosomal structures in <i>icd-1</i>(RNAi) embryos.

<p>Animals containing a <i>glo-1</i>::GFP expression vector were treated with <i>icd-1</i>(RNAi) for 48–56 hours and the resulting embryos were scored for the presence of large lysosomal structures in their gut cells. GLO-1 is a marker for <i>C. elegans</i> lysosomes that are also distinguishable by bio-fluorescence under polarized light. A majority of the embryos (n = 20) displayed <i>glo-1</i>::GFP signal coincident with lysosomal structures. A–D) A representative [<i>glo-1</i>::GFP] <i>icd-1</i>(RNAi) embryo visualized by A) fluorescent light; B) fluorescent light and DIC; C) fluorescent light and polarized DIC and D) polarized DIC. White arrows indicate lysosomes visualized by DIC with overlapping <i>glo-1</i>::GFP signal, red ovals outline the shell of the embryo.