Recombinant mYEL1, mAG5, or both in equal amounts were run by SDS-PAGE under non-reducing conditions. Western blot analysis was performed for four human sera samples anti-recombinant proteins positive in ELISA (exposed) and one negative control serum (NC). AB stands for recombinant protein strip stained by amidoblack. Molecular weights (kDa) of standard (STD; BenchMark Protein Ladder, ThermoFisher Scientific) are indicated.</p
<p>The five bat sera (bat serum #46, 48, 49, 50 and 53, respectively) were tested at 1∶100. The posi...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>(A) The recombinant Gly m 4l protein induced with 0.5 mM IPTG at 37°C for 1–5 h in <i>E</i>.<i>co...
<p>Serum was adsorbed against protein lysates from the <i>E</i>. <i>coli</i> expression vector and H...
<p>M: protein molecular weight markers; lane 1: MT separated with 150 mM imidazole; lane 2: MT separ...
<p>SDS-PAGE of recombinant proteins (panels A, B, and C, Coomassie Blue stained) or native (panel D,...
In some patients with plasma cell dyscrasia and neuropathy, there are IgM M proteins that react with...
(A) Coomassie brilliant blue staining. Western blot analysis of recombinant fusion proteins by (B) a...
<p>Reaction with the whole mAb V5B2 is compared to reactions with murine scFv (moScFv) and humanized...
<p>FL-PS-K and FL-PS-E were separated by SDS-PAGE under reducing conditions, transferred to a membra...
<p>(A) Coomassie brilliant blue (CBB) staining of recombinant AmJHAMT. Samples were: lane 1 (negativ...
Comparison of antibody reaction of human sera from Sudan, Ethiopia and both localities together agai...
<p>(A) Reducing 12.5% SDS-PAGE reference gel for (B to D) western blots. The reference gel was the s...
<p>100 ng of each recombinant protein was loaded on 15% polyacrylamide gels and analyzed as describe...
<p>Sera were in 1∶20 dilutions. Lane 1: negative control, lane 2: positive control, lanes 3–5: sampl...
<p>The five bat sera (bat serum #46, 48, 49, 50 and 53, respectively) were tested at 1∶100. The posi...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>(A) The recombinant Gly m 4l protein induced with 0.5 mM IPTG at 37°C for 1–5 h in <i>E</i>.<i>co...
<p>Serum was adsorbed against protein lysates from the <i>E</i>. <i>coli</i> expression vector and H...
<p>M: protein molecular weight markers; lane 1: MT separated with 150 mM imidazole; lane 2: MT separ...
<p>SDS-PAGE of recombinant proteins (panels A, B, and C, Coomassie Blue stained) or native (panel D,...
In some patients with plasma cell dyscrasia and neuropathy, there are IgM M proteins that react with...
(A) Coomassie brilliant blue staining. Western blot analysis of recombinant fusion proteins by (B) a...
<p>Reaction with the whole mAb V5B2 is compared to reactions with murine scFv (moScFv) and humanized...
<p>FL-PS-K and FL-PS-E were separated by SDS-PAGE under reducing conditions, transferred to a membra...
<p>(A) Coomassie brilliant blue (CBB) staining of recombinant AmJHAMT. Samples were: lane 1 (negativ...
Comparison of antibody reaction of human sera from Sudan, Ethiopia and both localities together agai...
<p>(A) Reducing 12.5% SDS-PAGE reference gel for (B to D) western blots. The reference gel was the s...
<p>100 ng of each recombinant protein was loaded on 15% polyacrylamide gels and analyzed as describe...
<p>Sera were in 1∶20 dilutions. Lane 1: negative control, lane 2: positive control, lanes 3–5: sampl...
<p>The five bat sera (bat serum #46, 48, 49, 50 and 53, respectively) were tested at 1∶100. The posi...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>(A) The recombinant Gly m 4l protein induced with 0.5 mM IPTG at 37°C for 1–5 h in <i>E</i>.<i>co...