Preparation of the recombinant AmJHAMT protein expressed in <i>E. coli</i>.

<p>(A) Coomassie brilliant blue (CBB) staining of recombinant AmJHAMT. Samples were: lane 1 (negative control), crude supernatant from BL21(DE3)/pET28a (empty vector); lane 2, crude supernatant from BL21(DE3)/pET28a(+)/AmJHAMT; and lane 3, recombinant AmJHAMT (marked by arrow) purified from the product shown in lane 2. (B) Western blotting using polyclonal anti-AmJHAMT antibody. Samples are the same with A. Arrow indicates the recombinant AmJHAMT. M shows the protein ladder and the molecular weight of its single band is listed on the left.