Western blot demonstrating specific response to recombinant AM854 and AM936 in AM854/AM936 vaccinates.

<p>Serum was adsorbed against protein lysates from the <i>E</i>. <i>coli</i> expression vector and His-tagged LacZ prior to western blotting. Recombinant AM854 (54kDa), AM936 (36kDa) and as a negative control, AM1096 (100kDa), were used as antigen. There is no antibody response to any of the proteins prior to immunization (Pre-immune). After immunization and adsorption (Adsorbed), anti-AM854 and anti-AM936 antibody, but no antibody directed toward AM1096 or the fusion tags was detected. One representative animal, 146, is shown. A serum dilution of 1:100 was used in all western blots. The anti-V5 antibody at a dilution of 1:500 demonstrates the presence of recombinant protein in each well.