The confirmation of antibody specificity using Western blotting.

<p>The recombinant CD4 was detected with A) anti-FLAG Ab and B) anti-<i>Fugu</i> CD4 Ab. C) <i>Fugu</i> CD4-CHO cells and D) <i>Fugu</i> PBLs lysates were detected with anti-<i>Fugu</i> CD4 Ab. The position of molecular weight markers is indicated to the left of each Western blot. The arrow indicates the predicted size of <i>Fugu</i> CD4 protein. Cont, translated with control plasmid. Nt, the lysate of non-transfected CHO.