Effect of miR-2110 overexpression on BE(2)-C cell differentiation, survival and proliferation.

(A-B), 2,500 cells were transfected with control oligo (25 nM), miR-2110 mimic (25 nM) or treated with RA (0.5 μM) in triplicate in 96-well plates. Neurite outgrowth was measured every 12 hours for 4 days. A, Representative cell images analyzed to define neurites (pink) and cell body areas (yellow) after 4 days of transfection. B, Time-dependent effect of miR-2110 mimic and RA on neurite outgrowth. *, p C, Effect of miR-2110 mimic and RA treatment on protein expression levels of cell differentiation markers. Cells were transfected or treated with the indicated molecules at above. After 4 days, protein lysates were collected and differentiation markers βIII-tubulin, GAP43 and NSE were determined by Western blot with calnexin protein level as a loading control. D, Effect of miR-2110 mimic and RA on cell viability. Cells were transfected as above, and cell viability was measured after 4 days. *, p E-G), Effect of miR-2110 mimic and RA on colony formation of BE(2)-C cells. Shown are plate images (E) and quantification of the number (F) and size (G) of colonies. *, p < 0.05 compared to control.