Effects of miR-615-5p overexpression on cell proliferation, colony formation, and apoptosis in pancreatic PANC-1 and MIA PaCa-2 cancer cell lines.

<p>PANC-1 and MIA PaCa-2 cells were transiently transfected with the miR-615-5p miRNA mimic (miR-615-5p) or a control construct (NC). (A) Cell proliferation was measured by CCK-8 detection at 24, 48, 72, 96, and 120 hours after transfection both in MIA PaCa-2 and PANC-1 cells. (B) Cell proliferation was also determined by 5-ethynyl-2’-deoxyuridine (EdU) staining 48 hours after transfection (Scale bar: 200 μm). Red: EdU; Blue: DAPI. (C) Colony formation assay after 21 days in culture. (D) Cell apoptosis was determined by flow cytometry analysis using Annexin V/PI staining 48 hours after transfection. The data are shown as mean±SD. *<i>P</i><0.05 <i>vs</i>. untreated cells or NC group.