Effects of methyl-β-cyclodextrin and cytochalasin D on the synergistic enhancement of the production of IL-6 of peripheral blood monocytes by anti-Sm mAb and anti-RNP mAb.

Highly purified monocytes were cultured in the presence or absence of methyl-β-cyclodextrin (5mM) and cytochalasin D (10 μM) with various combination of anti-Sm mAb, anti-RNP mAb, control IgG1 or IgG3 (3 μg/ml). After 48 hours of incubation, the supernatants were assayed for IL-6. Mean values with standard deviation (error bars) of 2 different experiments with reproducible results are shown.