Effects of human anti-Sm and human anti-RNP on the production of IL-6 of peripheral blood monocytes.

Highly purified monocytes (1×106/ml) from 4 healthy individuals were cultured in the presence of various IgG fractions containing varying concentrations of human anti-Sm and human anti-RNP at a final concentration of 100 μg/ml IgG. Thus, each IgG preparation shown in Table 1 was added at 1:10 [volume/volume]. After 48 hours of incubation, the supernatants were assayed for IL-6. Mean values with standard deviation (Error bars) of 4 different experiments are shown. The solid bar indicate the sum of IL-6 production in the presence of anti-RNP (0.9 U/ml) alone plus that in the presence of anti-Sm (1.1 U/ml) alone. Statistical significance was analyzed using Paired t test.