Synergistic enhancement of production of proinflammatory cytokines of human peripheral blood monocytes by anti-Sm and anti-RNP antibodies - Fig 3

Synergistic effects of anti-Sm mAb and anti-RNP mAb on the production of IL-6 (A) and TNF-α (B) of peripheral blood monocytes. Highly purified monocytes were cultured with varying concentrations of anti-Sm mAb in the presence of anti-RNP mAb (1 μg/ml) or control IgG1 (1 μg/ml). After 48 hours of incubation, the supernatants were assayed for IL-6. Mean values with standard deviation (Error bars) of 5 different experiments are shown. The broken line indicate the sum of the IL-6 and TNF-α production in the presence of anti-RNP mAb alone (1 μg/ml) plus that in the presence of IgG1 (1 μg/ml) with various concentrations of anti-Sm mAb. Statistical significance was analyzed using Paired t test. * Significantly higher (p≤0.05) than the point of anti-Sm of 1 μg/ml in the broken line. * * Significantly higher (p≤0.005) than the point of anti-Sm of 2 μg/ml in the broken line.