(A) mono-DCs treated with IFN-γ or left untreated were labeled with Smethionine and stimulated with β-glucan, Pam2C, R848, and their combinations

After 18 h, supernatants were immunoprecipitated with anti–IL-12 p35, anti–IL-23 p19, or anti–IL-12/23 p40 mAbs and resolved in nonreducing SDS-PAGE. (B) Cytokine production was measured by ELISA in mono-DCs 18 h after stimulation with 10 ng/ml LPS+R848, 10 μg/ml β-glucan+R848, or 10 μg/ml zymosan+R848 in the presence or absence of 50 ng/ml Pam2C (each symbol represents a single donor). (C) mono-DCs were stimulated as indicated in B in the presence or absence of 0.5, 5, and 50 ng/ml Pam2C, and IL-12 p75 was measured by ELISA (mean ± SD from triplicate cultures). (D) IL-12 p75 production was assessed by ELISA (mean ± SD from triplicate cultures) in mono-DCs stimulated with 10 μg/ml zymosan+R848 or 10 μg/ml β-glucan+R848 without or with 2, 20, and 200 ng/ml Pam3C; 1, 10, and 100 ng/ml MALP-2; or 0.5, 5, and 50 ng/ml Pam2C. (E) mono-DCs were stimulated with10 μg/ml zymosan+R848 (left) or with 0.1 μg/ml β-glucan+R848 (right) in the absence (open column) or presence (gray column) of 30 μg/ml of the neutralizing anti–IL-10 mAb 19F1, with or without 50 ng/ml Pam2C. Cytokines were measured by ELISA (mean values from duplicate cultures in a representative experiment of six performed with similar results).<p><b>Copyright information:</b></p><p>Taken from "Differential regulation of interleukin 12 and interleukin 23 production in human dendritic cells"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1447-1461.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413040.</p><p>