Mono-DCs treated with IFN-γ were labeled with Smethionine (A and B) or unlabeled (C and D) and stimulated with different combinations of MDP, Pam2C, and Pam3C, with or without R848

After 18 h, supernatants were immunoprecipitated with anti–IL-12 p35, anti–IL-23 p19, or anti–IL-12/23 p40 mAbs and resolved in nonreducing SDS-PAGE (A and B), or were evaluated for IL-12 p75, IL-23, and IL-10 by ELISA (C). In D, cells were lysed at 3, 6, and 12 h, and mRNA accumulation was determined using the QuantiGene multiplex assay. Results in A and B are representative of those obtained in three independent experiments with cells derived from different donors. Data in C are mean ± SE values from mono-DCs derived from three different donors. Results in D were obtained with cells from two different donors and are expressed as the mean ± SD of triplicate cultures. Inverted triangles indicate not done.<p><b>Copyright information:</b></p><p>Taken from "Differential regulation of interleukin 12 and interleukin 23 production in human dendritic cells"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1447-1461.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413040.</p><p>