MOESM5 of Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum

Additional file 5: Figure S2. Confirmation of deletion of 11 candidate genes derived from ∆PoxKu70 and the complementary strain. a PCR analysis. M, 1-kb DNA marker; lanes 1–3, three transformants for each candidate gene; lane 4, ∆PoxKu70; and lane 5, ddH2O. The top panel shows amplification of the region to the left of the target gene, the middle panel shows amplification of the region to the right of the target gene, and the bottom panel shows amplification of the region of the target gene. b Southern hybridization analysis of the deletion mutant ∆POX01907. M, 1-kb DNA marker; lane 1, ∆PoxKu70; lane 2, ∆POX01907-7; lane 3, ∆POX01907-9; and lane 4, ∆POX01907-15. c PCR confirmation of the complementary strain CPOX01907. M, 1-kb DNA marker; lane 1, CPOX01907; lane 2, ∆PoxKu70; and lane 3, ddH2O. The top panel shows amplification of the bleomycin-resistance gene, and the bottom panel shows amplification of complementary cassette