MOESM2 of The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilus

Additional file 2: Figure S1. Confirmation analysis of the deletion mutants of 23 candidate genes in T. pinophilus mutant ∆TpKu70 as the parental strain. A–W. PCR confirmation analysis of (A) ∆TP00297, (B) ∆TP02310, (C) ∆TP02980, (D) ∆TP03450, (E) ∆TP03988, (F) ∆TP05236, (G) ∆TP05746, (H) ∆TP05940, (I) ∆TP06128, (J) ∆TP06213, (K) ∆TP06945, (L) ∆TP06973, (M) ∆TP07409, (N) ∆TP08445, (O) ∆TP08615, (P) ∆TP08885, (Q) ∆TP09107, (R) ∆TP09505, (S) ∆TP09510, (T) ∆TP09544, (U) ∆TP09568, (V) ∆TP095904, (W) ∆TP12095. Line M: 1-kb DNA marker, Lanes 1–3: three transformants constructed for each candidate gene, Lane 4: ∆PoxKu70, Lane 5: ddH2O. The PCR products for each deletion mutant included the production of each target gene (Top), the production of the fragment on the left of the target gene (Middle) and the production of the fragment on the right of the target gene. X. Southern hybridization confirmation of the mutant ∆TP06128. The probe was amplified from the 5′-flanking sequence of TP06128. Lane M: 1-kb DNA marker, Lane 1: genomic DNA from ∆PoxKu70 as the control, Lanes 2–4, genomic DNA from three transformants of ∆TP06128