Additional file 3: of The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation

Figure S3. Effect of pH on RhoA activation in (A) THP-1 cells and (B) primary human CD14+ monocytes. Description of data: To confirm pH dependent RhoA activity, THP-1 cells and CD14+ monocytes were subjected to different pH (10 min) after a preliminary starvation step (2 h) at non-activating pH (pH 7.6) to silence the receptor. pH 6.6 elicited a significant increase in RhoA activation compared to pH 6.2, 7.4 and 7.6. GPR65/G12/13/RhoA signalling exhibits the highest activity at pH 6.6. (DOCX 65 kb