Additional file 6: of The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation

Figure S4. Formation of cAMP in human CD14+ monocytes upon pH shift from pH 7.6 to pH 6.5. Description of data: (A) Baseline values pH 7.6 and (B) after 10 min at acidic pH (pH 6.5). Human CD14+ cells were obtained from IBD patients carrying either rs8005161 TT, CT or WT/CC genotype, and non-IBD control subjects - all WT/CC genotype. 10 μM of G protein-coupled receptor 65 (GPR65) antagonist was used (C, D). No significant differences between the genotypes were identified. These data are identical to Fig. 1 but presented without normalization. cAMP: cyclic adenosine monophosphate, IBD: inflammatory bowel disease, WT: Wild type. (DOCX 403 kb