Additional file 2: of The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation

Figure S2. Effect of pH on cAMP formation in (A) THP-1 cells and primary human CD14+ monocytes isolated from (B) healthy subjects. To confirm that the pH values were associated with the activation and inactivation of GPR65/cAMP G-protein mediated signalling a pH dose response curve was generated. THP-1 cells and primary CD14+ human monocytes (WT/CC) were starved at pH 7.6 for 2 h (to silence the receptor) and subsequently subjected to a pH shift for 10 min (pH 6.2 to 7.8 with 0.2 increments). The highest cAMP accumulation was observed at pH 6.4–6.8, whereas low cAMP concentrations were demonstrated at pH 7.6–7.8. (DOCX 674 kb