Narya’s localization to discrete foci is dependent on DSB formation, and the number of Narya^GFP foci do not increase in early-mid pachytene in the absence of DSB repair.

(A) Early-mid pachytene (Region 2B) oocytes stained with C(3)G (magneta) to mark the SC and GFP (green) to mark Narya’s localization in the presence (a) or absence of DSBs (b and c). Genotypes are (a) naryaGFPcrispr; mei-W68/+, (b) naryaGFPcrispr; mei-W68 and (c) vilya naryaGFPcrispr. Images are maximum-intensity projections of the deconvolved z-series through the selected nuclei. Scale, 1 μm. (B) Early-mid pachytene (Region 2B) oocytes stained with Cona (blue) to mark the SC, γH2AV (red) to mark the DSBs, and GFP (green) to mark Narya in (a) naryaGFPcrispr; +/+ or (b) naryaGFPcrispr; okra. The average number of Narya foci in (a) was 5.4 (n = 12) and in (b) was 5.0 (n = 6), with (n) being the number of pro-oocytes scored in Region 2B. Images are maximum-intensity projections of the deconvolved z-series through the selected nuclei. Scale, 1 μm.