Aged mated and unmated germlines maintain DSB-2 localization in early pachytene.

A) Representative images of germlines stained with DSB-2. Solid lines indicate the “DSB-2 zone”, defined as the region of the germline in which >50% of nuclei are stained with DSB-2. Dashed lines extend from the end of the DSB-2 zone to the most proximal nucleus which has DSB-2 staining. Scale bars represent 20μm. B) Line plot representing the quantification of DSB-2 staining in young, aged mated, and aged unmated N2 hermaphrodite germlines. For specific maintenance schemes of these groups, see Fig 1A and Methods. Each horizontal line represents the portion of a single germline which contains DSB-2 positive nuclei. Solid lines represent the “DSB-2 zone”, while dashed lines extend to the most proximal germline position at which 1 or more nuclei is marked with DSB-2. C-D) Violin plots comparing the end of the DSB-2 zone and the final position of DSB-2 positive nuclei in young, aged mated, and aged unmated germlines. P values were calculated by Mann-Whitney U test with Bonferroni correction for multiple comparisons. Numerical data associated with this figure is presented in S7 Data. (TIF)