Narya localizes to sites of DSBs in both nurse cells and pro-oocytes.

An early pachytene 16-cell cyst from the genotype naryaGFPcrispr stained with antibodies to Cona (blue) to mark the pro-oocytes, γH2AV (red) to mark the DSBs and GFP (green) to mark Narya. The dashed box indicates two nuclei that are enlarged in the bottom row. One is a nurse cell (SC-negative) and the other is one of the pro-oocytes within the cyst (SC-positive). Scoring the SC-positive pro-oocytes at early pachytene, we found that 93% of the Narya foci colocalized with γH2AV foci (10 pro-oocytes scored). Genotype of the image shown is naryaGFPcrispr; +/Sp. Images are maximum-intensity projections of the deconvolved z-series through the selected nuclei. Scale, 1 μm.