DSBs in Oocytes Heterozygous for Three Balancer Chromosomes

<div><p>(A) Representatives from the oocytes with normal or heterozygous balancer chromosomes. Maximum intensity projection of image stacks of nuclei showing C(3)G (red) in conjunction with DNA (blue, DAPI staining) and DSB (green, γ-HIS2AV staining). Bars = 1 μm.</p><p>(B) Distribution graph representing the number of γ-HIS2AV foci per C(3)G staining nucleus at different developmental regions in germaria. The sample size of oocytes in wildtype is 64, 34, and 9, representing region 2a, 2b, and 3; in <i>FM7/+;SM1/+;TM3/+</i>, 48, 43, and 11; in <i>+/+;SM1/+;TM3/+</i>, 66, 38, and 10, respectively. Data are presented as means ± SD.</p>