<i>BSU1-EYFP BSK3-HA</i> plants exhibit increased <i>BSU1</i> transcript and protein levels.

(A) Twenty-two-day-old plants. Transgenic plants hemizygous for the 35Spro:BSU1-eYFP and BSK3pro:BSK3-HA transgenes were analyzed. Scale bar = 1 cm. (B) Semi-quantitative RT-PCR and western blot analyses of BSU1-EYFP and BSK3-HA gene and protein expression. One hundred micrograms of total proteins from 7-day-old light-grown seedlings were loaded. Transgenic plants homozygous for the BSK3pro:BSK3-HA transgene and hemizygous for the 35Spro:BSU1-EYFP transgene were analyzed. (C) Seven-day-old light-grown seedlings grown on ½ LS agar plates with or without BL. Scale bar = 2.5 mm. (D) Western blot analyses of BSU1-EYFP and BES1 protein expression in seedlings shown in (C). Fifty micrograms of total proteins were loaded. Asterisk (*) indicates the dephosphorylated BES1 protein.