Expression analysis of PpCBF3 and phenotype of WT and transgenic lines under normal and cold treatment conditions.

<p>(A) Shows 4-week-old seedlings of WT and transgenic lines grow under normal condition. (B) Phenotype of WT and transgenic lines after -20°C freezing stress. Red tick represents the stable transformed lines finally selected for further analysis. (C) Percentage of chlorotic leaves is calculated in (B). More than half yellow color of one leaf is designated as chlorolic leaf. Values are means ± SD of fifteen independent plants. Different letters on the top of bars indicate significant differences (<i>P</i> < 0.05) between WT and transgenic lines under the same growth condition. (D) Detection of the relative mRNA expression level of <i>PpCBF3</i> in WT and transgenic lines grow under normal condition. (E) PpCBF3-GFP fused protein is detected by western blot in transgenic lines but not in WT.