TPR deletion BSK3 protein fully rescues the <i>bsk3-1</i> mutant phenotypes.

(A) The structures of full-length (FL) and TPR deletion (TPR-Δ) BSK3 proteins. BSK3 has three tandem TPR motifs predicted by TPRpred. (B) Western blot analyses of BSK3-HA and BSK3TPR-Δ-HA protein expression. Twenty-five micrograms of total proteins from 7-day-old light-grown seedlings were loaded. (C) Primary root length of 7-day-old light-grown seedlings grown on ½ LS agar plates with or without 0.1 μM BL. Error bars represent SEM (n = 45–67). Different letters above the bars indicate significant differences (P Nicotiana benthamiana leaves. Scale bar = 100 μm. (E) Western blot analyses of transiently expressed proteins in Nicotiana benthamiana leaves. Twenty-five micrograms of microsomal proteins were loaded. Unknown proteins stained with Ponceau S are shown as loading controls.