<i>BSK3-HA</i> plants exhibit increased BR responses and defective shoot organ separation.

(A) Seven-day-old etiolated seedlings grown on ½ LS agar plates with or without 2 μM BRZ. Scale bar = 4 mm. (B) Hypocotyl length of 7-day-old etiolated seedlings. Error bars represent STD (n = 22–38). (C) Primary root length of 7-day-old light-grown seedlings. Error bars represent STD (n = 56–71). (D) Western blot analyses of BSK3-HA and BES1 protein expression. Asterisk (*) indicates the dephosphorylated BES1 protein. (E) Shoot organ fusion phenotypes of bzr1-1D, bes1-1D, and BSK3-HA plants. Arrows point to the fusion of cauline leaf and inflorescence stem. (F) Twenty-four-day-old plants and 7-day-old etiolated seedlings. bin2-1 was genotyped by PCR using bin2-1-FW and bin2-1-RV primers. Col-0 PCR fragments have a XhoI site, while bin2-1 PCR fragments do not have this site. Scale bar = 1 cm. (G) Hypocotyl length of 7-day-old etiolated seedlings. Error bars represent STD (n = 61–83). (H) Fifty-day-old plants and siliques. Scale bar = 4 cm (plants) or 2 mm (siliques). (D and F) Thirty micrograms of total proteins were loaded. (F-H) F3 or F4 bin2-1 plants homozygous for the BSK3pro:BSK3-HA transgene were analyzed. (B, C, and G) Different letters above the bars indicate significant differences (P < 0.05).