Western blot analysis of caspase activation.

(A) shRNA-GADD45γ and shRNA-NC HRE cells were incubated with 25 μM cisplatin for 24 hours. (B) shRNA-GADD45γ and shRNA-NC HRE cells were incubated with 25 μg/ml CsA for 24–48 hours. (C) HRE cells were infected with 50 MOI of adenoviral vectors and then treated with cisplatin at concentrations 25, 50, and 200 μM for 6 hours. Ad-LacZ served as controls. C-caspases represent cleaved (active)-caspases. shRNA-GADD45γ represents shRNA constructs to knockdown GADD45γ; shRNA-NC, shRNA constructs targeting no known genes. Ad-GADD45γ represents adenoviral vectors expressing GADD45γ; Ad-LacZ, LacZ adenoviral vectors.