Clustering display of the microarray data after cisplatin treatment.

<p>(A) RNA was extracted using an RNA isolation kit for microarray analysis after cisplatin treatment for 6 h. The up-regulated genes were functionally classified based on their biological process using the DAVID functional annotation clustering tool. (B) The mRNA levels of cell-death-associated genes in KYSE140 cells treated with 10 µM cisplatin for 6 h were determined by real-time RT-PCR. The data represent the mean ± SEM of relative mRNA levels versus untreated cells. (C) Protein levels of FOS, RIPK3, MLKL, Cytochrome c, survivin and caspase-9 in KYSE140 cells after treatment with 10 µM cisplatin for the indicated time points were analyzed by western blotting. (D) The mRNA levels of cell-death-associated genes in KYSE140 and EC0156 cells treated with 10 µM cisplatin for 6 h were determined by real-time PCR. **, <i>P</i><0.05; ***, <i>P</i><0.01.