Western blot analysis of apoptotic markers.

<p>Effects of WNT10A on cell survival were evaluated by western blot analysis of apoptotic markers. pcDNA-WNT10A-transfected 786-O showed a slightly reduced basal level of cleaved caspase 3 and PARP as compared to that observed in vector-transfected controls after 48 h of solvent (DMSO) treatment. However, β-catenin siRNA-transfected 786-O showed higher levels of cleaved caspase 3 and PARP than those observed in other groups of cells. After 48 h of treatment with 2 µM epirubicin or 10 µM cisplatin, pcDNA-WNT10A-transfected 786-O showed lower levels of cleaved caspase 3 and PARP than those observed in vector-transfected controls or cells cotransfected with pcDNA-WNT10A and β-catenin siRNA. Conversely, both WNT10A siRNA- and β-catenin siRNA-transfected Caki-1 showed higher levels of cleaved caspase 3 and PARP than those observed in scrambled siRNA-transfected controls. β-catenin silencing seemed to induce the production of cleaved caspase 3 and PARP. Both WNT10A siRNA- and β-catenin siRNA-transfected Caki-1 showed significantly higher levels of cleaved caspase 3 and PARP than those observed in scrambled siRNA-transfected controls after 48 h of treatment with 2 µM epirubicin or 10 µM cisplatin.