( and ) Western blot analysis of PARP cleaved form (89 kDa) protein expression (a) or Caspase 3 cleaved form (17 kDa) protein expression (b) performed in untreated A549, JFD18 or JFD9 cells (0) and in cells treated with 10 μM 12459 for 24 or 48 h

<p><b>Copyright information:</b></p><p>Taken from "Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence"</p><p>Nucleic Acids Research 2005;33(7):2192-2203.</p><p>Published online 14 Apr 2005</p><p>PMCID:PMC1079972.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> β-actin was used as a control for protein loading. Relative PARP cleavage (a) or Caspase 3 cleavage (b) was measured by densitometry scanning of the films, normalized relative to β-actin protein expression and results were expressed relative to untreated A549 cells defined as 100% for PARP or relative to A549 cells treated for 48 h with 12459 for caspase 3 (see bottom of each panel). () Effect of caspase inhibitors on apoptosis induced by 12459 (10 μM, 48 h) on A549 cells. DEVD-FMK and IETD-FMK at 2 μM were added to 12459 and apoptosis was revealed by Hoechst 33342 staining. () Mitochondrial membrane potential was measured by spectrofluorimetry of the JC1 dye in A549 control cells (0) or in cells treated with 1 μM 12459 for 0, 6, 12 or 24 h or with 10 μM 12459 for 4 or 6 h, as described in Materials and Methods. () ROS were measured by spectrofluorimetry using carboxy fluorescein-AM in A549 control cells (0) or in cells treated with 10 μM 12459 for 2, 4, 6, 8 and 14 h. Results represent the mean ± SM of duplicate determinations