WNT10A promotes wound healing ratio in RCC cells.

<p>After 48 h of transfection with 3 µg pcDNA-WNT10A or WNT10A siRNA, cells were scraped with p200 tip (time 0) and photographed each day. Forced WNT10A expression in 786-O and A498 increased the migration ability of these cells in the wound healing assay, especially 48 h after scraping. Moreover, cotransfection of pcDNA-WNT10A and β-catenin siRNA reduced WNT10A promotive effect on cell migration (<i>P</i><0.001, Student’s <i>t</i>-test between pcDNA-WNT10A-transfected cells and other cell groups). Conversely, each WNT10A siRNA knockdown in Caki-1 reduced the migration ability of these cells in the wound healing assay (<i>P</i><0.001 at 48 h after scraping, Student’s <i>t</i>-test between WNT10A siRNA-transfected cells and scrambled siRNA-transfected controls or between WNT10A siRNA-transfected cells and reagent-transfected controls). Wound repair percentage of each cell line is shown using bar charts.