TUNEL assay and cleaved caspase 3 to evaluate apoptosis in NRK-52E cells exposed to cisplatin and ALA + Fe.

<p>(A) TUNEL assay to evaluate apoptosis (green) in NRK-52E cells exposed to cisplatin or control. Nuclei were stained with propidium iodide (PI) (red). TUNEL assay to evaluate apoptosis (green) in NRK-52E cells exposed to cisplatin + ALA or cisplatin + ALA + Fe. (B)The induction of the number of apoptotic cells by cisplatin was reduced by ALA and ALA + Fe using quantitative analysis. Data are presented as mean ± SEM, n = 6. *P<0.05 vs. pcDNA-transfected cells. (C) Western blot analysis of cleaved caspase3 were performed following incubation with in control condition, cisplatin, cisplatin + ALA, and cisplatin + ALA + Fe. (D) Quantitative densitometry was performed for cleaved caspase 3 blots. Data are presented as mean ± SEM, n = 6. *P<0.05 by ANOVA.