Table S1. PCR primer sequences for 8 genes. Table S2. Mis-assignment rate of control group with Q30 filter. Table S3. Total contamination rates for each pooling scenario in Fig. 3a. Table S4. Total reads and rates of all WGS libraries (indexes 1â8). Table S5. Percentage of DNA sharing the same barcode with neighbours. Table S6. Effect of Q30 filter on sequencing reads and rates when library pooling is performed after PCR amplification (indexes 1â8). Table S7. Index contamination in water control with PCR-free library. Table S8. Raw data of PCR-free library contamination, 3 lanes. (XLSX 138 kb
Effect of laboratory and sequencing center variables on the probability of contamination, with the m...
Table S3. Sample pooling in two different sequencing runs according to final library concentration m...
Table S1. Large-Panel NGS Microsatellite Loci. Table S2. Detail on Samples, Targeted Sequencing, Mic...
Supplementary materials. Supplementary materials include Supplementary Tables and Figures. Figure S1...
Supplementary Figures S1-S13. Figure S1. Comparison of mutant allele fraction (MAF) in diluted sampl...
Figure S1. Schematic overview of the computational pipeline to identify somatic mutations in NG-TAS ...
Systematic approach to study exome capture variability in exome-sequencing (A) Three-generation pedi...
Table S7. Mean coverage achieved by Agilent SureSelect and Roche NimbleGen libraries within 90 PCR-c...
Found and missed SNPs out of the 66 CAPS-associated intra-individual SNPs from 62 single traces [22]...
Figure S3. Quality control information. A) DNA quantification pre-library-prep, but post-host-DNA-de...
DNA profiles in H3K4me3 and H3K27me3 ChIP-seq libraries generated through different purification rea...
Figure S1. Additional analyses of DNA sequencing data. (A) Plot showing the global read coverage of ...
Additional file 2: Fig. S1. Sequencing depth and rarefaction curves. Fig. S2. Performance of WGA in ...
Figure S2. Removal of contaminant sequence variants (SVs). a) Frequency plot of percentage of reads ...
Comparison of the metrics of sequence yield and quality of 18-h and 26-h WGS (HiSeq 2500 2 × 100 nt ...
Effect of laboratory and sequencing center variables on the probability of contamination, with the m...
Table S3. Sample pooling in two different sequencing runs according to final library concentration m...
Table S1. Large-Panel NGS Microsatellite Loci. Table S2. Detail on Samples, Targeted Sequencing, Mic...
Supplementary materials. Supplementary materials include Supplementary Tables and Figures. Figure S1...
Supplementary Figures S1-S13. Figure S1. Comparison of mutant allele fraction (MAF) in diluted sampl...
Figure S1. Schematic overview of the computational pipeline to identify somatic mutations in NG-TAS ...
Systematic approach to study exome capture variability in exome-sequencing (A) Three-generation pedi...
Table S7. Mean coverage achieved by Agilent SureSelect and Roche NimbleGen libraries within 90 PCR-c...
Found and missed SNPs out of the 66 CAPS-associated intra-individual SNPs from 62 single traces [22]...
Figure S3. Quality control information. A) DNA quantification pre-library-prep, but post-host-DNA-de...
DNA profiles in H3K4me3 and H3K27me3 ChIP-seq libraries generated through different purification rea...
Figure S1. Additional analyses of DNA sequencing data. (A) Plot showing the global read coverage of ...
Additional file 2: Fig. S1. Sequencing depth and rarefaction curves. Fig. S2. Performance of WGA in ...
Figure S2. Removal of contaminant sequence variants (SVs). a) Frequency plot of percentage of reads ...
Comparison of the metrics of sequence yield and quality of 18-h and 26-h WGS (HiSeq 2500 2 × 100 nt ...
Effect of laboratory and sequencing center variables on the probability of contamination, with the m...
Table S3. Sample pooling in two different sequencing runs according to final library concentration m...
Table S1. Large-Panel NGS Microsatellite Loci. Table S2. Detail on Samples, Targeted Sequencing, Mic...